DNAscent

Overview

DNAscent is software designed to detect the modified base BrdU in Oxford Nanopore reads. In an experimental setup where BrdU is incorporated into nascent DNA by replication forks, this software can be used to answer questions that were traditionally answered by DNA fibre analysis.

DNAscent is under active development by the Boemo Group based in the Department of Pathology, University of Cambridge. We aim to push regular updates and improvements, and incorporating new functionality is an active area of our computational research.

Publications

Please cite the following publication if you use DNAscent for your research:

Boemo, MA. DNAscent v2: Detecting Replication Forks in Nanopore Sequencing Data with Deep Learning. bioRxiv 2020. [Preprint DOI]

Muller CA, Boemo MA, Spingardi P, Kessler, BM, Kriaucionis S, Simpson JT, Nieduszynski CA. Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads. Nature Methods 2019;16:429-436. [Journal DOI]

Bugs, Questions, and Comments

Should any bugs arise or if you have any questions about usage, please raise a GitHub issue. If you have comments or suggestions to improve the software or the documentation, please Email Michael Boemo at mb915@cam.ac.uk.