Release Notes
v4.0.1
This is a pre-release. It is possible (maybe even probable) that the deep learning model and other parts of the software will be re-tuned in the coming weeks as we listen to community feedback.
All DNAscent executables now work on Oxford Nanopore R10.4.1 pores.
DNAscent detect
detects the thymidine analogues BrdU and EdU in single molecules as in v3.0.2 and v3.1.2.POD5 is not yet supported but POD5 support is planned for v4.0.2 which will be the LTS release. POD5 can be converted to FAST5 using the
pod5 convert to_fast5
utility (https://pypi.org/project/pod5/#pod5-convert-to_fast5).DNAscent’s deep learning models were trained on R10.4.1 flow cells with 5kHz sampling which is now the default sampling rate on Oxford Nanopore platforms. Using DNAscent with reads sequenced with the older 4kHz sampling rate is not recommended.
DNAscent index
still uses the sequencing_summary.txt file from Guppy and legacy versions of Guppy are available on the Oxford Nanopore Community webpage. Compatibility with Dorado is planned for v4.0.2 LTS.Tensorflow updated to 2.12.0 and, correspondingly, GPU usage now requires CUDA 11.8 and cuDNN 8.9.
Useage is otherwise identical to v3.1.2.
Training data for this release was provided by Mathew Jones at the University of Queensland. This software was developed in collaboration with the Jones Lab, Merrick Lab, and McClelland Lab to whom we are grateful for their collaboration and support. We are particularly grateful to them for supporting the release of the software to the community ahead of publication.
v3.1.2
DNAscent forkSense
now assigns a stall score to each called fork,DNAscent forkSense
can assign a replication stress signature to each called fork,DNAscent detect
no longer outputs the reference 6mer corresponding to each thymidine position in order to reduce output file size,improvements to fork calling and segmentation,
v3.0.2
DNAscent detect
now detects two different thymidine analogues, BrdU and EdU, in the same molecule,DNAscent forkSense
now uses the spatial patterning of EdU and BrdU to determine fork direction as in DNA fibre,dnascent2bedgraph utility updated to plot both EdU and BrdU tracks in genome browsers,
DNAscent regions
is now deprecated and has been fully superceded byDNAscent forkSense
,DNAscent psl
is now deprecated as reads can be more comprehensively plotted using the dnascent2bedgraph utility,Migration from Tensorflow 1.14 to 2.4.1 and, correspondingly, GPU usage now requires CUDA 11 and cuDNN 8,
v2.0.0
Migration from HMM-based BrdU detection at every thymidine to ResNet-based detection at every thymidine,
Significant increases to BrdU detection accuracy,
Support for BrdU detection on GPUs,
DNAscent forkSense
to call replication origins and termination sites in both synchronously and asynchronously replicating cells at any point in S-phase,DNAscent align
to align nanopore signals to reference,Significant increases to replication origin calling accuracy,
Visualisation utility for plotting output of multiple DNAscent executables as bedgraphs,
Released with Boemo, MA. DNAscent v2: Detecting replication forks in nanopore sequencing data with deep learning. BMC Genomics 2021;22:430.
v1.0.0
HMM-based BrdU detection at every thymidine,
Improvements to BrdU detection accuracy,
DNAscent train
to train Guassian mixture models from nanopolish eventalign.
v0.1
HMM-based BrdU detection at ~160 thymidine-containing 6mers,
Assignment of high- and low-BrdU regions based on Z-score,
Replication origin calling for early S-phase cells,